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Coauthors
Bastian Grewe Ruhr-University Bochum
2 protocols

Bianca Hoffmann
  • Department of Molecular and Medical Virology, Ruhr-University Bochum, Germany
Contributions
  • 2 Author merit

Education

M.Sc. in Biochemistry, Department of Molecular and Medical Virology, Ruhr-University Bochum, Germany, 2009

Current position

Ph.D. student, Department of Molecular and Medical Virology, Ruhr-University Bochum, Germany

Publications

  1. Grewe, B., Hoffmann, B., Ohs, I., Blissenbach, M., Brandt, S., Tippler, B., Grunwald, T. and Uberla, K. (2012). Cytoplasmic utilization of human immunodeficiency virus type 1 genomic RNA is not dependent on a nuclear interaction with gag. J Virol 86(6): 2990-3002.
  2. Grewe, B., Ehrhardt, K., Hoffmann, B., Blissenbach, M., Brandt, S. and Uberla, K. (2012). The HIV-1 Rev protein enhances encapsidation of unspliced and spliced, RRE-containing lentiviral vector RNA. PLoS ONE 7(11): e48688.
  3. Blissenbach, M., Grewe, B., Hoffmann, B., Brandt, S. and Uberla, K. (2010). Nuclear RNA export and packaging functions of HIV-1 Rev revisited. J Virol 84(13): 6598-6604.
2 Protocols published
Packaging of Retroviral RNA into Viral Particles Analyzed by Quantitative Reverse Transcriptase-PCR
Authors:  Bianca Hoffmann and Bastian Grewe, date: 04/20/2013, view: 7283, Q&A: 0
Formation of viral particles and packaging of genomic retroviral RNA into these particles are important steps in the late phase of the viral replication cycle. The efficiency of the incorporation of viral or cellular RNAs into viral particles can be ...
Detection and Cloning of Spliced Transcripts by RT-PCR
Authors:  Bianca Hoffmann and Bastian Grewe, date: 04/20/2013, view: 5681, Q&A: 0
Using a Reverse Transcriptase-PCR approach spliced transcripts can be converted to cDNA, amplified and cloned into an expression plasmid. Sequencing of the obtained cDNA allows identification of the splicing events that generated the detected RNA ...