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Coauthors
Søren Bak Faculty of Life Sciences - Department for Plant Biochemistry and Biotechnology, University of Copenhagen, Denmark
2 protocols

Carl Olsen Faculty of Life Sciences - Department of Basic Science and Environment, University of Copenhagen, Denmark
1 protocol

Reviewer
Jörg M. M. Augustin
  • University of Copenhagen
  • 2 Author merit

Education

Ph.D., Department of Plant Biology and Biochemistry, Faculty of Life Sciences, University of Copenhagen, Denmark, 2012

Current position

Postdoctoral Associate, Kutchan Lab, Donald Danforth Plant Science Center, St. Louis, USA

Publications

  1. Augustin, J. M., Drok, S., Shinoda, T., Sanmiya, K., Nielsen, J. K., Khakimov, B., Olsen, C. E., Hansen, E. H., Kuzina, V., Ekstrom, C. T., Hauser, T. and Bak, S. (2012). UDP-glycosyltransferases from the UGT73C subfamily in Barbarea vulgaris catalyze sapogenin 3-O-glucosylation in saponin-mediated insect resistance. Plant Physiol 160(4): 1881-1895.
  2. Augustin, J. M., Kuzina, V., Andersen, S. B. and Bak, S. (2011). Molecular activities, biosynthesis and evolution of triterpenoid saponins. Phytochemistry 72(6): 435-457.
  3. Kuzina, V., Nielsen, J. K., Augustin, J. M., Torp, A. M., Bak, S. and Andersen, S. B. (2011). Barbarea vulgaris linkage map and quantitative trait loci for saponins, glucosinolates, hairiness and resistance to the herbivore Phyllotreta nemorum. Phytochemistry 72(2-3): 188-198
2 Protocols published
Extraction and Reglucosylation of Barbarea vulgaris Sapogenins
Authors:  Jörg M. Augustin, Carl Erik Olsen and Søren Bak, date: 07/20/2013, view: 4086, Q&A: 0
Plants produce a vast array of natural compounds. Many of them are not commercially available, and are thus lacking to be tested as substrates for enzymes. This protocol describes the extraction and acidic hydrolysis of metabolites from Barbarea ...
Determination of Enzyme Kinetic Parameters of UDP-glycosyltransferases
Authors:  Jörg M. Augustin and Søren Bak, date: 07/20/2013, view: 4534, Q&A: 0
The determination of enzyme kinetic parameters, such as the Km and kcat values, is an essential part of the characterization of newly discovered enzymes. This protocol describes the determination of enzyme kinetic parameters of ...