James Lok Department of Pathobiology, University of Pennsylvania, USA
1 protocol

Hongguang Shao
  • Department of Pathobiology, University of Pennsylvania, USA
  • 1 Author merit


Ph.D. in Genetics, Institute of Genetics, Fudan University, Shanghai, P. R. China, 1997

Current position

Research Specialist D, Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, USA

Publications (since 2001)

  1. Shao, H., Li, X., Nolan, T. J., Massey, H. C., Jr., Pearce, E. J. and Lok, J. B. (2012). Transposon-mediated chromosomal integration of transgenes in the parasitic nematode Strongyloides ratti and establishment of stable transgenic lines. PLoS Pathog 8(8): e1002871.
  2. Li, X., Shao, H., Junio, A., Nolan, T. J., Massey, H. C., Jr., Pearce, E. J., Viney, M. E. and Lok, J. B. (2011). Transgenesis in the parasitic nematode Strongyloides ratti. Mol Biochem Parasitol 179(2): 114-119.
  3. Tordoff, M. G., Shao, H., Alarcon, L. K., Margolskee, R. F., Mosinger, B., Bachmanov, A. A., Reed, D. R. and McCaughey, S. (2008). Involvement of T1R3 in calcium-magnesium taste. Physiol Genomics 34(3): 338-348.
  4. Tordoff, M. G., Reed, D. R. and Shao, H. (2008). Calcium taste preferences: genetic analysis and genome screen of C57BL/6J x PWK/PhJ hybrid mice. Genes Brain Behav 7(6): 618-628.
  5. Shao, H., Reed, D. R. and Tordoff, M. G. (2007). Genetic loci affecting body weight and fatness in a C57BL/6J x PWK/PhJ mouse intercross. Mamm Genome 18(12): 839-851.
  6. Biedler, J. K.*, Shao, H.* and Tu, Z. (2007). Evolution and horizontal transfer of a DD37E DNA transposon in mosquitoes. Genetics 177(4): 2553-2558. (*equal contributors)
  7. Wilson, L., Ching, Y. H., Farias, M., Hartford, S. A., Howell, G., Shao, H., Bucan, M. and Schimenti, J. C. (2005). Random mutagenesis of proximal mouse chromosome 5 uncovers predominantly embryonic lethal mutations. Genome Res 15(8): 1095-1105.
  8. Holt, R. A., Subramanian, G. M., Halpern, A., Sutton, G. G., Charlab, R., Nusskern, D. R., Wincker, P., Clark, A. G., Ribeiro, J. M., Wides, R., Salzberg, S. L., Loftus, B., Yandell, M., Majoros, W. H., Rusch, D. B., Lai, Z., Kraft, C. L., Abril, J. F., Anthouard, V., Arensburger, P., Atkinson, P. W., Baden, H., de Berardinis, V., Baldwin, D., Benes, V., Biedler, J., Blass, C., Bolanos, R., Boscus, D., Barnstead, M., Cai, S., Center, A., Chaturverdi, K., Christophides, G. K., Chrystal, M. A., Clamp, M., Cravchik, A., Curwen, V., Dana, A., Delcher, A., Dew, I., Evans, C. A., Flanigan, M., Grundschober-Freimoser, A., Friedli, L., Gu, Z., Guan, P., Guigo, R., Hillenmeyer, M. E., Hladun, S. L., Hogan, J. R., Hong, Y. S., Hoover, J., Jaillon, O., Ke, Z., Kodira, C., Kokoza, E., Koutsos, A., Letunic, I., Levitsky, A., Liang, Y., Lin, J. J., Lobo, N. F., Lopez, J. R., Malek, J. A., McIntosh, T. C., Meister, S., Miller, J., Mobarry, C., Mongin, E., Murphy, S. D., O'Brochta, D. A., Pfannkoch, C., Qi, R., Regier, M. A., Remington, K., Shao, H., Sharakhova, M. V., Sitter, C. D., Shetty, J., Smith, T. J., Strong, R., Sun, J., Thomasova, D., Ton, L. Q., Topalis, P., Tu, Z., Unger, M. F., Walenz, B., Wang, A., Wang, J., Wang, M., Wang, X., Woodford, K. J., Wortman, J. R., Wu, M., Yao, A., Zdobnov, E. M., Zhang, H., Zhao, Q., Zhao, S., Zhu, S. C., Zhimulev, I., Coluzzi, M., della Torre, A., Roth, C. W., Louis, C., Kalush, F., Mural, R. J., Myers, E. W., Adams, M. D., Smith, H. O., Broder, S., Gardner, M. J., Fraser, C. M., Birney, E., Bork, P., Brey, P. T., Venter, J. C., Weissenbach, J., Kafatos, F. C., Collins, F. H. and Hoffman, S. L. (2002). The genome sequence of the malaria mosquito Anopheles gambiae. Science 298(5591): 129-149.
  9. Tu, Z. and Shao, H. (2002). Intra- and inter-specific diversity of Tc3-like transposons in nematodes and insects and implications for their evolution and transposition. Gene 282(1-2): 133-142.
  10. Shao, H. and Tu, Z. (2001). Expanding the diversity of the IS630-Tc1-mariner superfamily: discovery of a unique DD37E transposon and reclassification of the DD37D and DD39D transposons. Genetics 159(3): 1103-1115.
  11. Shao, H., Qi, Y. and Tu, Z. (2001). MsqTc3, a Tc3‐like transposon in the yellow fever mosquito Aedes aegypti. Insect molecular biology 10(5): 421-425
1 Protocol published
Detection of piggyBac-mediated Transposition by Splinkerette PCR in Transgenic Lines of Strongyloides ratti
Authors:  Hongguang Shao and James B. Lok, date: 01/05/2014, view: 5738, Q&A: 0
Splinkerette PCR (spPCR) is a newly developed and efficient method to ascertain and characterize genomic insertion sites of transgenes. The method described in this protocol was successfully applied to confirm piggyBac transposon-mediated ...