Search
Coauthors
Hailong Zhang Emory University
2 protocols

Muxiang Zhou
  • Department of Pediatrics, Emory University, USA
  • 2 Author merit
2 Protocols published
In vitro RNA-protein Binding Assay by UV Crosslinking
Authors:  Hailong Zhang and Muxiang Zhou, date: 11/05/2012, view: 11561, Q&A: 1
Because covalent bond can form between RNA and its binding proteins after UV irradiation, UV cross-linking is widely used to identify the specific RNA binding proteins. This protocol is described in details as follows.
Polysome Preparation, RNA Isolation and Analysis
Authors:  Hailong Zhang and Muxiang Zhou, date: 11/05/2012, view: 15772, Q&A: 2
During mRNA translation, 40S and 60S ribosomal subunits bind to target mRNA forming into an 80S complex (monosome). This ribosome moves along the mRNA during translational elongation to facilitate tRNA reading codon, where translation is activated ...