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Wei-Yun Winnie Wholey
  • Pediatrics and Communicable Diseases, University of Michigan, Medical School, USA
Contributions
  • 2 Author merit

Education

Ph.D. in Cellular and Molecular Biology, University of Michigan, Ann Arbor, MI, USA, 2012
M.S. in Microbiology, University of Massachusetts, Amherst, MA, USA, 2006

Current position

Postdoctoral researcher in Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, MI, USA

Publications

  1. Wholey, W. Y. and Jakob, U. (2012). Hsp33 confers bleach resistance by protecting elongation factor Tu against oxidative degradation in Vibrio cholerae. Mol Microbiol 83(5): 981-991.
  2. Blanchard, J. L., Wholey, W. Y., Conlon, E. M. and Pomposiello, P. J. (2007). Rapid changes in gene expression dynamics in response to superoxide reveal SoxRS-dependent and independent transcriptional networks. PLoS One 2(11): e1186.
2 Protocols published
Differential in vivo Thiol Trapping with N-ethylmaleimide (NEM) and 4-acetamido-4'-maleimidylstilbene-2,2'-disulfonic acid (AMS)
Author:  Wei-Yun (Winnie) Wholey, date: 10/20/2012, view: 7623, Q&A: 0
This protocol is used to compare the in vivo redox status of Escherichia coli and Vibrio cholerae protein before and after HOCl treatment. For example, I examined whether the EF-Tu protein is reduced or oxidized in the ...
Preparation of Genomic Overexpression Library
Author:  Wei-Yun (Winnie) Wholey, date: 10/05/2012, view: 5370, Q&A: 0
This protocol is used to identify/select for Escherichia coli genes that, when overexpressed in Vibrio cholerae Hsp33 (hslO gene) deletion mutant, protect against oxidative heat stress and, by extension, against HOCl-mediated ...