Arsalan Daudi University of California
1 protocol

Jose A. O'Brien
  • Royal Holloway University of London
Research focus
  • Plant science
  • 1 Author merit


Ph.D. in Biological Sciences, Royal Holloway University of London, UK, 2011

Current position

Postdoctoral Scientist in the Plant Systems Biology Lab, Department of Molecular Genetics and Microbiology, Universidad Catolica de Chile, Chile


  1. O'Brien, J. A. and Benkova, E. (2013). Cytokinin cross-talking during biotic and abiotic stress responses. Front Plant Sci 4: 451.
  2. O'Brien, J. A.*, Daudi, A.*, Butt, V. S. and Bolwell, G. P. (2012). Reactive oxygen species and their role in plant defence and cell wall metabolism. Planta 236(3): 765-779. (*Co-correspondence authors)
  3. Daudi, A. and O’Brien, J. A. (2012). Detection of hydrogen peroxide by DAB staining in Arabidopsis leaves. Bio-protocol 2(18): e263.
  4. Daudi, A., Cheng, Z., O'Brien, J. A., Mammarella, N., Khan, S., Ausubel, F. M. and Bolwell, G. P. (2012). The apoplastic oxidative burst peroxidase in Arabidopsis is a major component of pattern-triggered immunity. Plant Cell 24(1): 275-287.
  5. O'Brien, J. A., Daudi, A., Finch, P., Butt, V. S., Whitelegge, J. P., Souda, P., Ausubel, F. M. and Bolwell, G. P. (2012). A peroxidase-dependent apoplastic oxidative burst in cultured Arabidopsis cells functions in MAMP-elicited defense. Plant Physiol 158(4): 2013-2027.
1 Protocol published
Detection of Hydrogen Peroxide by DAB Staining in Arabidopsis Leaves
Authors:  Arsalan Daudi and Jose A. O’Brien, date: 09/20/2012, view: 38263, Q&A: 17
In this protocol, the in situ detection of hydrogen peroxide (one of several reactive oxygen species) is described in mature Arabidopsis rosette leaves by staining with 3,3'-diaminobenzidine (DAB) using an adaptation of previous ...