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Coauthors
Vanesa Olivares-Illana Instituto de Física, Universidad Autónoma de San Luis Potosí
2 protocols

Robin Farhaeus
  • Cibles Therapeutiques, INSERM Unite 940, Universite Paris 7, France
  • 2 Author merit
2 Protocols published
Purification of His-ubiquitin Proteins from Mammalian Cells
Authors:  Vanesa Olivares Illana and Robin Farhaeus, date: 09/05/2012, view: 10501, Q&A: 6
This protocol is used to purify His-tag ubiquitin conjugated protein. In this particular case, cells were transfected with His-tag ubiquitin and p53 which allowed us to purify using His-tag and reveal the WB using antibodies against p53 to see just ...
Protein-RNA ELISA Assay
Authors:  Vanesa Olivares Illana and Robin Farhaeus, date: 09/05/2012, view: 6181, Q&A: 0
Protein-RNA ELISA assay is an effective and quantitative method to study protein-RNA interactions in vitro. In this protocol we used recombinant 6x HIS tagged protein, but it works as well for non tagged proteins.