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Darran G. Cronshaw
  • Center for Autoimmune and Musculoskeletal Diseases, Feinstein Institute for Medical Research, USA
  • 2 Author merit
2 Protocols published
Transfection of S2 Cell with DNA Using CellFectin Reagent
Author:  Darran G. Cronshaw, date: 03/05/2012, view: 12633, Q&A: 0
This method provides a step-by-step guide to transfecting Drosophila S2 cells with the pRmHA-3 (or similar) vector with insert of choice (in this case SDF-1β-FLAG) and generating a stable cell line. This cell line is then capable of producing the ...
Calcium Mobilisation Assay in Response to Chemokine Stimulation
Author:  Darran G. Cronshaw, date: 05/05/2012, view: 7936, Q&A: 1
This assay is used to measure calcium mobilization in lymphocytes (either in primary cells or cell lines) in response to chemokine stimulation using ratiometric analysis. It has also been used for measuring TCR mediated calcium flux. In addition, ...