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Coauthors
Randy Schekman Department of Molecular and Cell Biology and Howard Hughes Medical Institute, University of California, USA, USA,
1 protocol

Kanika Bajaj Department of Molecular and Cell Biology and Howard Hughes Medical Institute, University of California, USA, USA,
1 protocol

Satoshi Baba Department of Molecular and Cell Biology and Howard Hughes Medical Institute, University of California, USA, USA,
1 protocol

Lin Yuan
  • Department of Molecular and Cell Biology and Howard Hughes Medical Institute, University of California, USA, USA,
  • 1 Author merit
1 Protocol published
Cell-free Generation of COPII-coated Procollagen I Carriers
Authors:  Lin Yuan, Satoshi Baba, Kanika Bajaj and Randy Schekman, date: 11/20/2017, view: 366, Q&A: 0
The aim of this protocol is to generate COPII-coated procollagen I (PC1) carriers in a cell-free reaction. The COPII-coated PC1 carriers were reconstituted from donor membrane, cytosol, purified recombinant COPII proteins, and nucleotides. This ...