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Coauthors
Caroline Dean John Innes Centre, Norwich Research Park, United Kingdom
1 protocol

Matthew Hartley John Innes Centre, Norwich Research Park, United Kingdom
1 protocol

Tjelvar Olsson John Innes Centre, Norwich Research Park, United Kingdom
1 protocol

Susan Duncan Earlham Institute, Norwich Research Park, United Kingdom
1 protocol
Coreviewers
Arsalan Daudi University of California
153 protocols

Eunsook Park Seoul National University
3 protocols

Pablo Bolanos-Villegas University of Costa Rica
9 protocols

Reviewer
Stefanie Rosa
  • University of Potsdam
Research focus
  • Cell biology
Contributions
  • 2 Author merit
  • 2 Reviewer merit

Publications

  1. Rosa, S., Duncan, S. and Dean, C. (2016). Mutually exclusive sense-antisense transcription at FLC facilitates environmentally induced gene repression. Nat Commun 7: 13031.
  2. Duncan, S., Olsson, T. S., Hartley, M., Dean, C. and Rosa, S. (2016). A method for detecting single mRNA molecules in Arabidopsis thaliana. Plant Methods 12: 13.
  3. Zhu, D., Rosa, S. and Dean, C. (2015). Nuclear organization changes and the epigenetic silencing of FLC during vernalization. J Mol Biol 427(3): 659-669.
  4. Rosa, S., Ntoukakis, V., Ohmido, N., Pendle, A., Abranches, R. and Shaw, P. (2014). Cell differentiation and development in Arabidopsis are associated with changes in histone dynamics at the single-cell level. Plant Cell 26(12): 4821-4833.
  5. Podufall, J., Tian, R., Knoche, E., Puchkov, D., Walter, A. M., Rosa, S., Quentin, C., Vukoja, A., Jung, N., Lampe, A., Wichmann, C., Böhme, M., Depner, H., Zhang, Y. Q., Schmoranzer, J., Sigrist, S. J. and Haucke, V. (2014). A presynaptic role for the cytomatrix protein GIT in synaptic vesicle recycling. Cell Rep 7(5): 1417-1425.
  6. Rosa, S., De Lucia, F., Mylne, J. S., Zhu, D., Ohmido, N., Pendle, A., Kato, N., Shaw, P. and Dean, C. (2013). Physical clustering of FLC alleles during Polycomb-mediated epigenetic silencing in vernalization. Genes Dev 27(17): 1845-1850.
  7. Rosa, S. and Shaw, P. (2013). Insights into chromatin structure and dynamics in plants. Biology (Basel) 2(4): 1378-1410.
  8. Pires, A., Rosa, S., Castanheira, S., Fevereiro, P. and Abranches, R. (2012). Expression of a recombinant human erythropoietin in suspension cell cultures of Arabidopsis, tobacco and Medicago. Plant Cell Tiss Org
2 Protocols published
Single Molecule RNA FISH in Arabidopsis Root Cells
Authors:  Susan Duncan, Tjelvar S. G. Olsson, Matthew Hartley, Caroline Dean and Stefanie Rosa, date: 04/20/2017, view: 1751, Q&A: 0
Methods that allow the study of gene expression regulation are continually advancing. Here, we present an in situ hybridization protocol capable of detecting individual mRNA molecules in plant root cells, thus permitting the accurate ...
Two-photon Photoactivation to Measure Histone Exchange Dynamics in Plant Root Cells
Authors:  Stefanie Rosa and Peter Shaw, date: 10/20/2015, view: 2316, Q&A: 0
Chromatin-binding proteins play a crucial role in chromatin structure and gene expression. Direct binding of chromatin proteins both maintains and regulates transcriptional states. It is therefore important to study the binding properties of these ...
2 Protocols reviewed
Direct Visualization and Quantification of the Actin Nucleation and Elongation Events in vitro by TIRF Microscopy
Authors:  Yuxiang Jiang and Shanjin Huang, date: 03/05/2017, view: 1462, Q&A: 0
Total internal reflection fluorescence (TIRF) microscopy is a powerful tool for visualizing the dynamics of actin filaments at single-filament resolution in vitro. Thanks to the development of various fluorescent probes, we can easily ...
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A Live-imaging, Heat Shock-inducible System to Measure Aux/IAA Degradation Rates in Planta
Authors:  Jessica M. Guseman, Jennifer L. Nemhauser and Britney L. Moss, date: 08/05/2016, view: 2865, Q&A: 0
An emerging theme in biology is the importance of cellular signaling dynamics. In addition to monitoring changes in absolute abundance of signaling molecules, many signal transduction pathways are sensitive to changes in temporal properties of ...
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