Education

Ph.D. in Science, Graduate School of Science, The University of Tokyo, 1983

Current position

Professor in Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Kashiwa, Chiba, Japan

Publication (since 2011)

1. Higaki, T., Kutsuna, N., Akita, K., Sato, M., Sawaki, F., Kobayashi, M., Nagata, N., Toyooka, K. and Hasezawa, S. (2015). Semi-automatic organelle detection on transmission electron microscopic images. Sci Rep 5: 7794.
   
2. Nomura, T., Itouga, M., Kojima, M., Kato, Y., Sakakibara, H. and Hasezawa, S. (2015). Copper mediates auxin signalling to control cell differentiation in the copper moss Scopelophila cataractae. J Exp Bot 66(5): 1205-1213.
   
3. Hirakawa, Y., Nomura, T., Hasezawa, S. and Higaki, T. (2015). Simplification of vacuole structure during plant cell death triggered by culture filtrates of Erwinia carotovora. J Integr Plant Biol 57(1): 127-135.
   
4. Fujiwara, T., Kawachi, M., Sato, Y., Mori, H., Kutsuna, N., Hasezawa, S. and Maeshima, M. (2015). A high molecular mass zinc transporter MTP12 forms a functional heteromeric complex with MTP5 in the Golgi in Arabidopsis thaliana. FEBS J 282(10): 1965-1979.
   
5. Takahashi, S., Kojo, K. H., Kutsuna, N., Endo, M., Toki, S., Isoda, H. and Hasezawa, S. (2015). Differential responses to high- and low-dose ultraviolet-B stress in tobacco Bright Yellow-2 cells. Front Plant Sci 6: 254.
   
6. Akita, K., Higaki, T., Kutsuna, N. and Hasezawa, S. (2015). Quantitative analysis of microtubule orientation in interdigitated leaf pavement cells. Plant Signal Behav 10(5): e1024396.
   
7. Higaki, T., Hashimoto-Sugimoto, M., Akita, K., Iba, K. and Hasezawa, S. (2014). Dynamics and environmental responses of PATROL1 in Arabidopsis subsidiary cells. Plant Cell Physiol 55(4): 773-780.
   
8. Kojo, K. H., Yasuhara, H. and Hasezawa, S. (2014). Time-sequential observation of spindle and phragmoplast orientation in BY-2 cells with altered cortical actin microfilament patterning. Plant Signal Behav 9.
   
9. Higaki, T., Kutsuna, N. and Hasezawa, S. (2014). CARTA-based semi-automatic detection of stomatal regions on an Arabidopsis cotyledon surface. Plant Morphology 26: 9-12.
   
10. Hasegawa, J., Higaki, T., Hamamura, Y., Kurihara, D., Kutsuna, N., Higashiyama, T., Hasezawa, S. and Matsunaga, S. (2014). Increase in invaginated vacuolar membrane structure cfaused by plant cell expansion by genotoxic stress induced by DNA double-strand breaks. Cytologia 79: 467-474.
    
11. Toyooka, K., Sato, M., Kutsuna, N., Higaki, T., Sawaki, F., Wakazaki, M., Goto, Y., Hasezawa, S., Nagata, N. and Matsuoka, K. (2014). Wide-range high-resolution transmission electron microscopy reveals morphological and distributional changes of endomembrane compartments during log to stationary transition of growth phase in tobacco BY-2 cells. Plant Cell Physiol 55(9): 1544-1555.
    
12. Higaki, T., Higaki, S., Hirota, M. and Hasezawa, S. (2014). Radiocesium distribution in bamboo shoots after the Fukushima nuclear accident. PLoS One 9(5): e97659.
    
13. Higaki, T., Kato, A., Myouga, F., Kutsuna, N., Hasezawa, S. and Nagata, N. (2014). Automatic classification of chloroplast ultrastructure mutants with transmission electron microscopy. Bioimages 22: 1-7.
    
14. Higaki, T., Kutsuna, N. and Hasezawa, S. (2013). LIPS database with LIPService: a microscopic image database of intracellular structures in Arabidopsis guard cells. BMC Plant Biol 13: 81.
    
15. Kojo, K. H., Higaki, T., Kutsuna, N., Yoshida, Y., Yasuhara, H. and Hasezawa, S. (2013). Roles of cortical actin microfilament patterning in division plane orientation in plants. Plant Cell Physiol 54(9): 1491-1503.
    
16. Murata, T., Sano, T., Sasabe, M., Nonaka, S., Higashiyama, T., Hasezawa, S., Machida, Y. and Hasebe, M. (2013). Mechanism of microtubule array expansion in the cytokinetic phragmoplast. Nat Commun 4: 1967.
    
17. Hashimoto-Sugimoto, M., Higaki, T., Yaeno, T., Nagami, A., Irie, M., Fujimi, M., Miyamoto, M., Akita, K., Negi, J., Shirasu, K., Hasezawa, S. and Iba, K. (2013). A Munc13-like protein in Arabidopsis mediates H+-ATPase translocation that is essential for stomatal responses. Nat Commun 4: 2215.
    
18. Kariya, K., Demiral, T., Sasaki, T., Tsuchiya, Y., Turkan, I., Sano, T., Hasezawa, S. and Yamamoto, Y. (2013). A novel mechanism of aluminum-induced cell death involving vacuolar processing enzyme and vacuolar collapse in tobacco cell line BY-2. J Inorg Biochem 128: 196-201.
    
19. Kojo, K. H., Higaki, T., Kutsuna, N., Yoshida, Y., Yasuhara, H. and Hasezawa, S. (2013). Roles of cortical actin microfilament patterning in division plane orientation in plants. Plant Cell Physiol 54(9): 1491-1503.
    
20. Akita, K., Hasezawa, S. and Higaki, T. (2013). Breaking of plant stomatal one-cell-spacing rule by sugar solution immersion. PLoS One 8(9): e72456.
    
21. Fujita, S., Pytela, J., Hotta, T., Kato, T., Hamada, T., Akamatsu, R., Ishida, Y., Kutsuna, N., Hasezawa, S., Nomura, Y., Nakagami, H. and Hashimoto, T. (2013). An atypical tubulin kinase mediates stress-induced microtubule depolymerization in Arabidopsis. Curr Biol 23(20): 1969-1978.
    
22. Era, A., Kutsuna, N., Higaki, T., Hasezawa, S., Nakano, A. and Ueda, T. (2013). Microtubule stability affects the unique motility of F-actin in Marchantia polymorpha. J Plant Res 126(1): 113-119.
    
23. Hirota, M., Higaki, S., Higaki, T. and Hasezawa, S. (2013). Investigation of contamination in spinach collected immediately following the Fukushima Daiichi nuclear disaster. Radiation Safety Management 12(2): 43-47.
    
24. Sano, T., Yoshihara, T., Handa, K., Sato, M. H., Nagata, T. and Hasezawa, S. (2012). Metal Ion Homeostasis Mediated by NRAMP Transporters in Plant Cells - Focused on Increased Resistance to Iron and Cadmium Ion. Crosstalk and Integration of Membrane Trafficking Pathway 213-228.
    
25. Higaki, T., Kutsuna, N., and Hasezawa, S. (2012). Probabilistic mapping and image clustering for quantitative assessment of fluorescent protein localizations in Arabidopsis guard cells. Protocol Exchange (Nature Publishing Group, invitation)
    
26. Higaki, T., Kutsuna, N., Hosokawa, Y., Akita, K., Ebine, K., Ueda, T., Kondo, N. and Hasezawa, S. (2012). Statistical organelle dissection of Arabidopsis guard cells using image database LIPS. Sci Rep 2: 405.
    
27. Yazawa, K., Iwai, H., Sugiyama, H., Ito, S., Hasezawa, S. and Satoh, S. (2012). Ectopic expression of the leucine-rich repeat-domain of cell wall extension gene NpLRX1 disturbs morphogenesis of leaf cells by its association with cell membranes in tobacco. Plant Biotech 12: 0612.
    
28. Kutsuna, N., Higaki, T., Matsunaga, S., Otsuki, T., Yamaguchi, M., Fujii, H. and Hasezawa, S. (2012). Active learning framework with iterative clustering for bioimage classification. Nat Commun 3: 1032.
    
29. Okubo-Kurihara, E., Higaki, T., Kurihara, Y., Kutsuna, N., Yamaguchi, J. and Hasezawa, S. (2011). Sucrose transporter NtSUT4 from tobacco BY-2 involved in plant cell shape during miniprotoplast culture. J Plant Res 124(3): 395-403.
    
30. Nomura, T. and Hasezawa, S. (2011). Regulation of gemma formation in the copper moss Scopelophila cataractae by environmental copper concentrations. J Plant Res 124(5): 631-638.
    
31. Higaki, T., Kurusu, T., Hasezawa, S. and Kuchitsu, K. (2011). Dynamic intracellular reorganization of cytoskeletons and the vacuole in defense responses and hypersensitive cell death in plants. J Plant Res 124(3): 315-324.