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    An Acute Mouse Spinal Cord Slice Preparation for Studying Glial Activation ex vivo
    Authors:  Juan Mauricio Garré, Guang Yang, Feliksas F. Bukauskas and Michael V. L. Bennett, date: 01/20/2017, view: 2213, Q&A: 0
    [Abstract] Pathological conditions such as amyotrophic lateral sclerosis, spinal cord injury and chronic pain are characterized by activation of astrocytes and microglia in spinal cord and have been modeled in rodents. In vivo imaging at cellular level in these animal models is limited due to the spinal cord’s highly myelinated funiculi. The ...
    Primary Culture of Mouse Neurons from the Spinal Cord Dorsal Horn
    Authors:  De-Li Cao, Peng-Bo Jing, Bao-Chun Jiang and Yong-Jing Gao, date: 01/05/2017, view: 2436, Q&A: 0
    [Abstract] Primary afferents of sensory neurons mainly terminate in the spinal cord dorsal horn, which has an important role in the integration and modulation of sensory-related signals. Primary culture of mouse spinal dorsal horn neuron (SDHN) is useful for studying signal transmission from peripheral nervous system to the brain, as well as for developing ...
    Microinjection of Virus into Lumbar Enlargement of Spinal Dorsal Horn in Mice
    Authors:  Zhi-Jun Zhang, Peng-Bo Jing and Yong-Jing Gao, date: 11/20/2016, view: 2574, Q&A: 0
    [Abstract] In order to explore the role of a specific gene/protein in the specific segment of the spinal cord, the technique of intraspinal injection is particularly used to deliver viral vectors targeting the specific gene/protein. These viral vectors can knockdown or overexpress the specific gene/protein in specific cells (glial cells or neurons). In this ...
    An in vitro Model of Neuron-macrophage Interaction to Generate Macrophages with Neurite Outgrowth Properties
    Authors:  Hyeok Jun Yun and Byung S. Kim, date: 11/20/2016, view: 1977, Q&A: 0
    [Abstract] Macrophages are known to play beneficial roles in axon regeneration after nerve injury. To develop an in vitro model in which injury signals can elicit pro-regenerative macrophage activation, we established co-cultures consisting of adult dorsal root ganglia sensory neurons and peritoneal macrophages and added cAMP analogue dibutyryl ...
    Organotypic Spinal Cord Slice Cultures and a Method to Detect Cell Proliferation in These Slices
    Authors:  Jillian M. Daniel, Jim Deuchars and Susan A. Deuchars, date: 10/05/2016, view: 2166, Q&A: 0
    [Abstract] In these culture models, the normal cytoarchitecture and local neuronal circuits of the spinal cord are preserved, offering a compromise between dissociated cell cultures and complete animal studies. The addition of 5-ethynyl-2’-deoxyuridine (EdU) to the culture medium allows for the detection of proliferating cells.
    Isolation and Primary Cell Culture of Mouse Dorsal Root Ganglion Neurons
    Authors:  Theresa Heinrich, Christian A. Hübner and Ingo Kurth, date: 04/05/2016, view: 5573, Q&A: 0
    [Abstract] We here provide a detailed protocol for the isolation and culture of primary mouse sensory neurons. The cell bodies of sensory afferent pseudounipolar neurons are located in dorsal root ganglia (DRGs) along the vertebral column. Dissected mouse DRGs can be dissociated into single cells by enzymatic digestion to obtain primary cultures of mouse ...
    Isolation and Growth of Adult Mouse Dorsal Root Ganglia Neurons
    Authors:  Seong-il Lee and Joel Levine, date: 09/20/2015, view: 8154, Q&A: 0
    [Abstract] Adult dorsal root ganglia neurons are among the few adult neuronal cell types that can be purified and grown relatively easily in dissociated cell culture. Here we describe a procedure for the isolation and growth of dissociated adult mouse DRG neurons using Percoll gradients and a chemically defined medium. These cultures can be used for ...