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    Determination of NO and CSF Levels Produced by Bacillus subtilis
    [Abstract] The cell-to-cell communication and division of labour that occurs inside a beneficial biofilm produce significant differences in gene expression compared with the gene expression pattern of cells grew under planktonic conditions. In this sense, the levels of NO (nitric oxide) and CSF (Competence Sporulation Stimulating Factor) produced in Bacillus ...
    Culturing Bacteria from Caenorhabditis elegans Gut to Assess Colonization Proficiency
    [Abstract] Determining an accurate count of intestinal bacteria from Caenorhabditis elegans is one critical way to assess colonization proficiency by a given bacteria. This can be accomplished by culturing appropriate dilutions of worm gut bacteria on selective or differential agarized media. Because of the high concentration of bacteria in gut ...
    CRISPR/Cas9 Editing of the Bacillus subtilis Genome
    Authors:  Peter E. Burby and Lyle A. Simmons, date: 04/20/2017, view: 2373, Q&A: 0
    [Abstract] A fundamental procedure for most modern biologists is the genetic manipulation of the organism under study. Although many different methods for editing bacterial genomes have been used in laboratories for decades, the adaptation of CRISPR/Cas9 technology to bacterial genetics has allowed researchers to manipulate bacterial genomes with ...
    Differentiation of Naturally Produced Extracellular Membrane Vesicles from Lipid Aggregation by Glucuronoxylomannan Immunogold Transmission Electron Microscopy in Bacillus subtilis
    Authors:  Lisa Brown, Geoff Perumal and Arturo Casadevall, date: 03/05/2015, view: 3439, Q&A: 0
    [Abstract] Recently, membrane vesicle (MV) production was described in Gram-positive bacteria, which harbor a variety of components such as toxins, antibiotic resistance proteins, proteases, DNA, and immune modulators. Free lipids have the ability to form micelles, thus it is important to rule out spontaneous association of lipids into vesicle-like ...
    Vesicle Isolation from Bacillus subtilis Biofilm
    Authors:  Lisa Brown, Anne Kessler and Arturo Casadevall, date: 03/05/2015, view: 4059, Q&A: 0
    [Abstract] Bacterial biofilms are associated clinically with many bacterial infections including those caused by bacteria such as Pseudomonas aeruginosa and Staphylococcus aureus. In recent years, extracellular vesicles produced by bacteria have been isolated from biofilm communities. Vesicles have been described in depth and can ...
    Isolation of the Secretome from Bacillus subtilis
    Authors:  Juri Niño Bach and Marc Bramkamp, date: 01/20/2014, view: 3968, Q&A: 0
    [Abstract] Bacteria are commonly known to secret proteins in large amounts into the surrounding environment in high concentrations via various pathways. These proteins can be involved in numerous processes like cell-cell communication, exopolymer formation but also metabolic active enzymes are secreted that are interesting for industrial production of ...
    Preparation of Bacillus subtilis Cell Lysates and Membranes
    Authors:  Juri Niño Bach and Marc Bramkamp, date: 01/20/2014, view: 6521, Q&A: 0
    [Abstract] A common feature of every eukaryotic and prokaryotic cell is that they exhibit a plasma membrane. In Bacillus subtilis (B. subtilis) roughly 25% of all proteins are putative trans- or membrane associated proteins. Here we describe a relatively simple method to separate and prepare membrane and cytosolic proteins by ...