Protocols in Current Issue
    Analysis of 3D Cellular Organization of Fixed Plant Tissues Using a User-guided Platform for Image Segmentation
    Authors:  Ethel Mendocilla Sato and Célia Baroux, date: 06/20/2017, view: 937, Q&A: 0
    [Abstract] The advent of non-invasive, high-resolution microscopy imaging techniques and computational pipelines for high-throughput image processing has contributed to gain insights in plant organ morphogenesis at the cellular level. Confocal scanning laser microscopy (CSLM) allows the generation of three dimensional images constituted of serial optical ...
    Use of SCRI Renaissance 2200 (SR2200) as a Versatile Dye for Imaging of Developing Embryos, Whole Ovules, Pollen Tubes and Roots
    Authors:  Thomas J. Musielak, Patrick Bürgel, Martina Kolb and Martin Bayer, date: 09/20/2016, view: 2404, Q&A: 0
    [Abstract] Confocal laser scanning microscopy in combination with fluorescent proteins is a powerful tool for the study of sexual reproduction and other developmental processes in plants. In order to understand the origin and localization of fluorescent signals in a complex tissue, staining of cell outlines is often mandatory. Cell wall staining with SCRI ...