Protocols in Current Issue
    Extraction of Soluble and Insoluble Protein Fractions from Mouse Brains and Spinal Cords
    Author:  Oliver Wirths, date: 08/05/2017, view: 415, Q&A: 0
    [Abstract] The current protocol details the preparation of soluble and insoluble protein lysates from mouse brain or spinal cord samples. In detail, tissue homogenization and sequential protein extraction are described. This procedure yields soluble and insoluble protein extracts that can be further processed in down-stream applications like ELISA or Western ...
    Contusion Spinal Cord Injury Rat Model
    Authors:  Chuan-Wen Chiu, Henrich Cheng and Shie-Liang Hsieh, date: 06/20/2017, view: 463, Q&A: 0
    [Abstract] Spinal cord injury (SCI) can lead to severe disability, paralysis, neurological deficits and even death. In humans, most spinal cord injuries are caused by transient compression or contusion of the spinal cord associated with motor vehicle accidents. Animal models of contusion mimic the typical SCI’s found in humans and these models are key to the ...
    Peripheral Nerve Injury: a Mouse Model of Neuropathic Pain
    Authors:  Takahiro Masuda, Yuta Kohro, Kazuhide Inoue and Makoto Tsuda, date: 05/05/2017, view: 885, Q&A: 0
    [Abstract] Neuropathic pain is one of the highly debilitating chronic pain conditions, for which, currently, there is no therapeutic treatment. In order to reveal the underlying mechanism for neuropathic pain, various animal models have been established (Burma et al., 2016). This protocol describes how to prepare spinal nerve injury model (Kim and ...
    An Acute Mouse Spinal Cord Slice Preparation for Studying Glial Activation ex vivo
    Authors:  Juan Mauricio Garré, Guang Yang, Feliksas F. Bukauskas and Michael V. L. Bennett, date: 01/20/2017, view: 1785, Q&A: 0
    [Abstract] Pathological conditions such as amyotrophic lateral sclerosis, spinal cord injury and chronic pain are characterized by activation of astrocytes and microglia in spinal cord and have been modeled in rodents. In vivo imaging at cellular level in these animal models is limited due to the spinal cord’s highly myelinated funiculi. The ...
    Primary Culture of Mouse Neurons from the Spinal Cord Dorsal Horn
    Authors:  De-Li Cao, Peng-Bo Jing, Bao-Chun Jiang and Yong-Jing Gao, date: 01/05/2017, view: 1837, Q&A: 0
    [Abstract] Primary afferents of sensory neurons mainly terminate in the spinal cord dorsal horn, which has an important role in the integration and modulation of sensory-related signals. Primary culture of mouse spinal dorsal horn neuron (SDHN) is useful for studying signal transmission from peripheral nervous system to the brain, as well as for developing ...
    Microinjection of Virus into Lumbar Enlargement of Spinal Dorsal Horn in Mice
    Authors:  Zhi-Jun Zhang, Peng-Bo Jing and Yong-Jing Gao, date: 11/20/2016, view: 2003, Q&A: 0
    [Abstract] In order to explore the role of a specific gene/protein in the specific segment of spinal cord, the technique of intraspinal injection is particularly used to deliver viral vectors targeting the specific gene/protein. These viral vectors can knockdown or overexpress the specific gene/protein in specific cells (glial cells or neurons). In this ...
    An in vitro Model of Neuron-macrophage Interaction to Generate Macrophages with Neurite Outgrowth Properties
    Authors:  Hyeok Jun Yun and Byung S. Kim, date: 11/20/2016, view: 1586, Q&A: 0
    [Abstract] Macrophages are known to play beneficial roles in axon regeneration after nerve injury. To develop an in vitro model in which injury signals can elicit pro-regenerative macrophage activation, we established co-cultures consisting of adult dorsal root ganglia sensory neurons and peritoneal macrophages and added cAMP analogue dibutyryl ...
    In vitro Brainstem-spinal Cord Preparation from Newborn Rat
    Authors:  Jean-Patrick Le Gal, Angelo Nicolosi, Laurent Juvin and Didier Morin, date: 11/20/2016, view: 1566, Q&A: 0
    [Abstract] The brainstem-spinal cord preparation of newborn rat contains neural networks able to produce motor output in absence of sensory feedback. These neural structures, commonly called central pattern generators (CPGs), are involved in many vital functions such as respiration (Morin and Viala, 2002; Giraudin et al., 2008) or locomotion (Juvin ...
    Organotypic Spinal Cord Slice Cultures and a Method to Detect Cell Proliferation in These Slices
    Authors:  Jillian M. Daniel, Jim Deuchars and Susan A. Deuchars, date: 10/05/2016, view: 1693, Q&A: 0
    [Abstract] In these culture models, the normal cytoarchitecture and local neuronal circuits of the spinal cord are preserved, offering a compromise between dissociated cell cultures and complete animal studies. The addition of 5-ethynyl-2’-deoxyuridine (EdU) to the culture medium allows for the detection of proliferating cells.
    Isolation and Primary Cell Culture of Mouse Dorsal Root Ganglion Neurons
    Authors:  Theresa Heinrich, Christian A. Hübner and Ingo Kurth, date: 04/05/2016, view: 4465, Q&A: 0
    [Abstract] We here provide a detailed protocol for the isolation and culture of primary mouse sensory neurons. The cell bodies of sensory afferent pseudounipolar neurons are located in dorsal root ganglia (DRGs) along the vertebral column. Dissected mouse DRGs can be dissociated into single cells by enzymatic digestion to obtain primary cultures of mouse ...