Cell Biology


    Protocols in Current Issue
    Measurement of Extracellular Ca2+ Influx and Intracellular H+ Efflux in Response to Glycerol and PEG6000 Treatments
    Authors:  Tao Li and Baodong Chen, date: 09/20/2013, view: 3895, Q&A: 0
    [Abstract] The characteristics of Ca2+ and H+ fluxes may reflect the activities of aquaporins, as the up-regulation of aquaporin activities is directly associated with the decrease in cytoplasmic H+ concentration and increase in cytoplasmic Ca2+ concentration. The higher aquaporin activities can protect cells ...
    Calcium Mobilization Assay to Measure the Activity of Gq-coupled Receptors
    Author:  Hamiyet Unal, date: 06/20/2013, view: 7809, Q&A: 0
    [Abstract] Calcium mobilization assay is a cell-based second messenger assay to measure the calcium flux associated with Gq-protein coupled receptor activation or inhibition. The method utilizes a calcium sensitive fluorescent dye that is taken up into the cytoplasm of most cells. In some cell lines in which organic-anion transporters are particularly active ...
    Measurement of Free Cytosolic Calcium Concentration ([Ca2+]i) in Single CHO-K1 Cells
    Authors:  Manuel D. Gahete, Raúl M. Luque and Justo P. Castaño, date: 11/20/2012, view: 5895, Q&A: 0
    [Abstract] This is a protocol to analyze the functional response of single CHO-K1 cells to a given treatment in terms of changes in free cytosolic calcium concentration ([Ca2+]i). This is possible by using the Ca2+ indicator dye Fura-2 AM, a polyamino carboxylic acid that binds to free intracellular calcium and is excited at 340 nm and 380 nm. The ratio of ...
    Calcium Mobilisation Assay in Response to Chemokine Stimulation
    Author:  Darran G. Cronshaw, date: 05/05/2012, view: 7626, Q&A: 1
    [Abstract] This assay is used to measure calcium mobilization in lymphocytes (either in primary cells or cell lines) in response to chemokine stimulation using ratiometric analysis. It has also been used for measuring TCR mediated calcium flux. In addition, the same labeling procedure with the addition of brilliant black (a quenching agent) (Sigma-Aldrich, ...
    [Bio101] Calcium Imaging
    Author:  Mingye Feng, date: 04/05/2012, view: 21030, Q&A: 4
    [Abstract] This is a protocol of Ca2+ imaging experiment using Ca2+ indicator Fura-2. Ca2+ imaging is an efficient and quantitative method for measuring cytosolic and internal store Ca2+ levels, as well as their dynamic changes.