Plant Science


    Protocols in Current Issue
    Using Silicon Polymer Impression Technique and Scanning Electron Microscopy to Measure Stomatal Aperture, Morphology, and Density
    Authors:  Hui-Chen Wu, Ya-Chen Huang, Chia-Hung Liu and Tsung-Luo Jinn, date: 08/20/2017, view: 409, Q&A: 0
    [Abstract] The number of stomata on leaves can be affected by intrinsic development programming and various environmental factors, in addition the control of stomatal apertures is extremely important for the plant stress response. In response to elevated temperatures, transpiration occurs through the stomatal apertures, allowing the leaf to cool through ...
    Root Aliphatic Suberin Analysis Using Non-extraction or Solvent-extraction Methods
    Authors:  Camille Delude, Sollapura J. Vishwanath, Owen Rowland and Frédéric Domergue, date: 06/20/2017, view: 623, Q&A: 0
    [Abstract] Here we describe both non-extraction and solvent-extraction methods for root aliphatic suberin analysis. The non-extraction method is fast as roots are directly depolymerized using acidic transmethylation. However, suberin aliphatic components are isolated together with all the other acyl chains making up the lipids (e.g., membranes) ...
    Determining Genome Size from Spores of Seedless Vascular Plants
    Authors:  Li-Yaung Kuo and Yao-Moan Huang , date: 06/05/2017, view: 692, Q&A: 0
    [Abstract] Seedless vascular plants, including ferns and lycophytes, produce spores to initiate the gametophyte stage and to complete sexual reproduction. Approximately 10% of them are apomictic through the production of genomic unreduced spores. Being able to measure the spore nuclear DNA content is therefore important to infer their reproduction mode. Here ...
    Documentation of floral secretory glands in Pleurothallidinae (Orchidaceae) using Scanning Electron Microscopy (SEM)
    Authors:  Adam P. Karremans, Bertie Joan van Heuven, Rob Langelaan and Barbara Gravendeel, date: 11/20/2016, view: 1628, Q&A: 0
    [Abstract] A clear, step by step description of the treatment of orchid flowers, subtribe Pleurothallidinae, with Critical Point Drying for SEM is presented. It shows that a simple, short fixation and dehydration method prior to Critical Point Drying is sufficient to obtain good results.
    Cell Wall-bound p-Coumaric and Ferulic Acid Analysis
    Author:  Nickolas Anderson, date: 10/05/2016, view: 1267, Q&A: 0
    [Abstract] Hydroxycinnamic acids, such as p-coumaric acid and ferulic acid, are a major class of compounds derived from the phenylpropanoid pathway. These compounds are widely conserved in plants and primarily accumulate in the secondary cell wall. They serve as important structural components that contribute to the overall strength and rigidity of ...
    Sample Preparation for X-ray Micro-computed Tomography of Woody Plant Material and Associated Xylem Visualisation Techniques
    Author:  Nicholas J. B. Brereton, date: 03/20/2016, view: 2117, Q&A: 0
    [Abstract] Variation in the tissue structure of short rotation coppice (SRC) willow is a principle factor driving differences in lignocellulosic sugar yield yet much of the physiology and development of this tissue is unknown. Traditional sectioning can be both difficult and destructive in woody tissue; however, technology such as three dimensional X-ray ...
    Extraction of Intracellular and Cell Wall Proteins from Leaves and Roots of Harsh Hakea
    Authors:  Michael W. Shane and William C. Plaxton, date: 12/05/2015, view: 1936, Q&A: 0
    [Abstract] Plant proteins can be targeted to intracellular (i.e., cytosol, vacuole, organelles etc.) or extracellular (i.e., cell walls, apoplast) compartments. Dual targeting is a key mechanism with important implications for plant metabolism, growth, development and defense etc. Harsh Hakea (Hakea prostrata ...
    Detection and Visualization of Specific Gene Transcripts by in situ RT-PCR in Nematode-Infected Arabidopsis Root Tissue
    Author:  Krzysztof Wieczorek, date: 09/20/2015, view: 2414, Q&A: 0
    [Abstract] This protocol describes an effective method of in situ RT-PCR that was developed to localize specific gene expression directly in thin cross-sections of nematode feeding sites induced by the cyst nematode Heterodera schachtii (H. schachtii) or the root-knot nematode Meloidogyne incognita (M. incognita) ...
    Transverse Sectioning of Arabidopsis thaliana Leaves Using Resin Embedding
    Authors:  Shweta Kalve, Kumud Saini, Kris Vissenberg, Tom Beeckman and Gerrit T. S. Beemster, date: 09/20/2015, view: 3880, Q&A: 0
    [Abstract] The leaf is the major functional part of the shoot performing the bulk of photosynthetic activity. Its development is relatively plastic allowing the plant to adapt to environmental changes by modifying leaf size and anatomy. Moreover, a leaf is made up of various distinct cell layers, each having specialized functions. To understand functional ...
    Tissue Culturing and Harvesting of Protonemata from the Moss Physcomitrella patens
    Authors:  Xiaoqin Wang and Yikun He, date: 08/05/2015, view: 2628, Q&A: 0
    [Abstract] Moss spores germinate to form an alga-like filamentous structure called the protonemata. Protonemata are the earliest stage (the haploid phase) of a bryophyte life cycle and eventually give rise to a mature gametophyte. Protonemata of the moss Physcomitrella patens (P. patens) are important not only in their life cycle, but also ...