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    Xanthomonas oryzae pv. oryzae Inoculation and Growth Rate on Rice by Leaf Clipping Method
    Authors:  Yinggen Ke, Shugang Hui and Meng Yuan, date: 10/05/2017, view: 681, Q&A: 0
    [Abstract] Bacterial blight caused by Xanthomonas oryzae pv. oryzae (Xoo) is one of the most serious bacterial diseases and a major impediment to the increase of rice yield. Appropriate methods for inoculation of Xoo and disease scoring are necessary to investigate the nature of the disease and the mechanism of plant ...
    Wheat Coleoptile Inoculation by Fusarium graminearum for Large-scale Phenotypic Analysis
    Authors:  Lei-Jie Jia, Wan-Qiu Wang and Wei-Hua Tang, date: 08/05/2017, view: 831, Q&A: 0
    [Abstract] The ascomycete fungus Fusarium graminearum is a destructive fungal pathogen of wheat, barley and maize. Although reverse genetics and homologous recombination gene deletion methods have generated thousands of gene deletion mutants of F. graminearum, evaluating virulence of these fungal mutants is still a rate-limiting step. Here ...
    Isolation of Ustilago bromivora Strains from Infected Spikelets through Spore Recovery and Germination
    Authors:  Jason Bosch and Armin Djamei, date: 07/20/2017, view: 867, Q&A: 0
    [Abstract] Ustilago bromivora is a biotrophic smut fungus infecting Brachypodium sp. It is closely related to the barley-infecting smut Ustilago hordei, and related to the well-studied, gall-inducing model pathogen Ustilago maydis. Upon flowering, the spikelets of U. bromivora-infected plants are filled with black ...
    Exopolysaccharide Quantification for the Plant Pathogen Ralstonia solanacearum
    Authors:  Rémi Peyraud, Timothy P. Denny and Stéphane Genin, date: 05/20/2017, view: 1252, Q&A: 0
    [Abstract] Soluble exopolysaccharide is a major virulence factor produced by the plant pathogen Ralstonia solanacearum. Its massive production during plant infection is associated with the arrest of water flow in xylem vessels leading eventually to plant death. The composition of this heavy macromolecule includes mainly N-acetylgalactosamine. Here ...
    Pathogenicity Assay of Penicillium expansum on Apple Fruits
    Authors:  Yong Chen, Boqiang Li, Zhanquan Zhang and Shiping Tian, date: 05/05/2017, view: 1362, Q&A: 0
    [Abstract] Penicillium expansum, a widespread filamentous fungus, is a major causative agent of fruit decay and leads to huge economic losses during postharvest storage and shipping. Furthermore, it produces mycotoxin on the infected fruits that may cause harmful effects to human health. This pathogenicity assay involves a stab inoculation procedure ...
    Growth Assay for the Stem Parasitic Plants of the Genus Cuscuta
    Authors:  Volker Hegenauer , Max Welz, Max Körner and Markus Albert, date: 04/20/2017, view: 1355, Q&A: 0
    [Abstract] Cuscuta spp. are widespread obligate holoparasitic plants with a broad host spectrum. Rootless Cuscuta penetrates host stems with so called haustoria to form a direct connection to the host vascular tissue (Dawson et al., 1994; Lanini and Kogan, 2005; Kaiser et al., 2015). This connection allows a steady uptake ...
    Wheat Root-dip Inoculation with Fusarium graminearum and Assessment of Root Rot Disease Severity
    Authors:  Qing Wang and Sven Gottwald, date: 03/20/2017, view: 1767, Q&A: 0
    [Abstract] Fusarium graminearum is one of the most common and potent fungal pathogens of wheat (Triticum aestivum) and other cereals, known for causing devastating yield losses and mycotoxin contaminations of food and feed. The pathogen is mainly considered as a paradigm for the floral disease Fusarium head blight, while its ability to ...
    Pathogenicity Assay of Verticillium nonalfalfae on Hop Plants
    Authors:  Marko Flajšman, Sebastjan Radišek and Branka Javornik, date: 03/20/2017, view: 1589, Q&A: 0
    [Abstract] Verticillium nonalfalfae is a soil-borne plant pathogen that infects its hosts through roots. It spreads in the plant’s xylem and causes wilt disease symptoms by secreting different virulence factors. Hop (Humulus lupulus) is a primary host of V. nonalfalfae, so it is used as a model plant for studying this ...
    Penetration Assays, Fungal Recovery and Pathogenicity Assays for Verticillium dahliae
    Authors:  Yun-Long Zhao, Tao Zhang and Hui-Shan Guo, date: 02/20/2017, view: 2000, Q&A: 0
    [Abstract] Verticillium dahliae is a soil-borne phytopathogenic fungus that infects host roots and proliferates in vascular tissues. The great loss of economically important crop caused by V. dahliae has raised worldwide concern, however, little is known about the mechanism of its pathogenicity (Klosterman et al., 2011; Yadeta and ...
    Bioassay of Xanthomonas albilineans Attachment on Sugarcane Leaves
    Authors:  Imène Mensi, Jean-Heinrich Daugrois and Philippe Rott, date: 01/20/2017, view: 2050, Q&A: 0
    [Abstract] Sugarcane (interspecific hybrids of Saccharum species) is an economically important crop that provides 70% of raw table sugar production worldwide and contributes, in some countries, to bioethanol and electricity production. Leaf scald, caused by the bacterial plant pathogen Xanthomonas albilineans, is one of the major diseases ...