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    Protocols in Current Issue
    Monitoring Xylem Hydraulic Pressure in Woody Plants
    [Abstract] Xylem sap circulates under either positive or negative hydraulic pressure in plants. Negative hydraulic pressure (i.e., tension) is the most common situation when transpiration is high, and several devices have been developed to quantify it accurately (e.g., Scholander pressure chamber, psychrometers). However, a proper ...
    Assessing Plant Tolerance to Acute Heat Stress
    Authors:  Minsoo Kim, Fionn McLoughlin, Eman Basha and Elizabeth Vierling, date: 07/20/2017, view: 658, Q&A: 0
    [Abstract] It is well-established that plants are able to acclimate to temperatures above or below the optimal temperature for their growth. Here, we provide protocols for assays that can be used quantitatively or qualitatively to assess the relative ability of plants to acquire tolerance to high temperature stress. The hypocotyl elongation assay described ...
    Forest GPP Calculation Using Sap Flow and Water Use Efficiency Measurements
    Authors:  Fyodor Tatarinov, Eyal Rotenberg, Dan Yakir and Tamir Klein, date: 04/20/2017, view: 1024, Q&A: 0
    [Abstract] This is a protocol to evaluate gross primary productivity (GPP) of a forest stand based on the measurements of tree’s sap flow (SF), 13C derived water use efficiency (WUE), and meteorological (met) data. GPP was calculated from WUE and stomatal conductance (gs), the later obtained from SF up-scaled from sampled trees to stand ...
    Establishment of New Split-root System by Grafting
    Authors:  Xiangqiang Kong, Zhen Luo and Hezhong Dong, date: 02/20/2017, view: 1311, Q&A: 0
    [Abstract] A new split-root system was used to simulate non-uniform salt, drought or nutrient deficiency stress in the root zone, in which the root system was divided into two or more equal portions. Here, we established a split-root system by grafting of cotton seedlings. In contrast to the conventional split-root, the main roots of the new system remained ...
    A Method to Analyze Local and Systemic Effects of Environmental Stimuli on Root Development in Plants
    Authors:  Neil E. Robbins II and José Dinneny, date: 09/05/2016, view: 2262, Q&A: 0
    [Abstract] Root development in vascular plants is innately tied to the environment. However, relatively little attention has been paid toward understanding the spatial scales at which the root perceives and responds to external stimuli. While some environmental signals elicit global responses that affect root system architecture, others may have more ...
    Measurements of Proline and Malondialdehyde Contents and Antioxidant Enzyme Activities in Leaves of Drought Stressed Cotton
    Authors:  Tianzi Chen and Baolong Zhang, date: 09/05/2016, view: 2558, Q&A: 1
    [Abstract] Drought stress negatively affects cotton plant growth and induces various biochemical and physiological responses in cotton plants. Proline content and antioxidant enzymes are thought to be associated with maintaining the structure of cellular components or with protecting cellular function. Study of cotton plant responses towards drought stress ...
    Salinity and Drought Treatment Assays in Kenaf (Hibiscus cannabinus L.)
    Authors:  Xiaoping Niu, Meixia Chen, Aifen Tao and Jianmin Qi, date: 09/05/2016, view: 1762, Q&A: 0
    [Abstract] Salinity and drought are the two main factors that cause fiber yield and quality losses in kenaf. It is reported that salinity and drought can affect more than 10% of arable land and cause a global decline in the average yields of major crops by more than 50%. Therefore, understanding plant tolerance of drought and salinity is of fundamental ...
    Experimental Design to Determine Drought Stress Response and Early Leaf Senescence in Barley (Hordeum vulgare L.)
    Authors:  Gwendolin Wehner, Christiane Balko and Frank Ordon, date: 03/05/2016, view: 3300, Q&A: 0
    [Abstract] Premature leaf senescence induced by drought stress is a main factor for yield losses in barley. Research in drought stress tolerance has become more important as due to climate change the number of drought periods will increase and tolerance to drought stress has become a goal of high interest in barley breeding. However, reliable screening for ...
    Cotyledon Wounding of Arabidopsis Seedlings
    Authors:  Debora Gasperini, Ivan F. Acosta and Edward E. Farmer, date: 01/20/2016, view: 2872, Q&A: 0
    [Abstract] Damage to plant organs through both biotic and abiotic injury is very common in nature. Arabidopsis thaliana 5-day-old (5-do) seedlings represent an excellent system in which to study plant responses to mechanical wounding, both at the site of the damage and in distal unharmed tissues. Seedlings of wild type, transgenic or mutant lines ...
    Quantifying the Permeability of the Apoplastic Water Barrier in Cosmos Petals
    Authors:  Reinhard Jetter and Christopher Buschhaus, date: 11/20/2015, view: 1788, Q&A: 0
    [Abstract] The capacity of plants to minimize uncontrolled water loss is essential for survival in adverse and changing climatic conditions. In order to assess and compare the effectiveness of apoplastic barriers to water, the permeability of the barrier must first be quantified. Studies have accomplished this directly by quantifying tritium flux or ...