Plant Science


    Protocols in Current Issue
    Measurement of ATP Hydrolytic Activity of Plasma Membrane H+-ATPase from Arabidopsis thaliana Leaves
    Authors:  Masaki Okumura and Toshinori Kinoshita, date: 12/05/2016, view: 1608, Q&A: 0
    [Abstract] Plant plasma membrane H+-ATPase, which is a P-type ATPase, couples ATP hydrolysis to H+ extrusion and thereby generates an electrochemical gradient across the plasma membrane. The proton gradient is necessary for secondary transport, cell elongation, and membrane potential maintenance. Here we describe a protocol for ...
    Cytohistochemical Determination of Calcium Deposition in Plant Cells
    Authors:  Wan-Jun Zhang and Tao Wang, date: 01/20/2016, view: 1757, Q&A: 0
    [Abstract] Calcium plays important roles in maintaining plant cellular structure and also acts as a key secondary messenger in intercellular signaling. Thirty years ago, methods of detecting calcium in sub-cellular level had been established (Stockwell and Hanchey, 1982; Borgers et al., 1982) and reviewed extensively (Wick and Heplerm, 1982). We had ...
    Measurement of H+ flux in Rice by Non-invasive Micro-test Technology
    Authors:  Xi Zhang, Xiu-Yue Zhang and Li-Ping Yin, date: 12/05/2015, view: 1845, Q&A: 0
    [Abstract] Rice plants release proton (H+) from root cells into rhizosphere area leading to the acidification of the rhizosphere and increased solubility of ferric iron complexes on the cell membrane, which is important for iron uptakes. Here, we present a detailed protocol to measure H+ flux in root hairs of transgenic rice seedlings ...
    An Assay to Test the Capacity of Arabidopsis Plant Defensin Type1 Protein to Induce Cellular Zinc (Zn) Tolerance in Yeast
    Authors:  Zaigham Shahzad, Laurence Marquès, Pierre Berthomieu and Françoise Gosti, date: 11/20/2015, view: 2138, Q&A: 0
    [Abstract] Heterologous expression of genes in budding yeast Saccharomyces cerevisiae (S. cerevisiae) is especially suitable to functionally study the corresponding encoded protein at the cellular level (Bonneaud et al., 1991). This is mainly because many strains defective in specific activities are available and could be ...
    Histochemical Staining of Silica Body in Rice Leaf Blades
    [Abstract] Silicon (Si) is a biologically important element for plants in the order Poales (Yamamoto et al., 2011; Kido et al., 2015). In rice, Si is mainly deposited in the motor cells and the cell walls of the leaf epidermis. However, the molecular basis of this overall process has not been elucidated. Thus, we propose a protocol for the ...
    High-throughput Quantification of Ammonium Content in Arabidopsis
    Authors:  Izargi Vega-Mas, Asier Sarasketa and Daniel Marino, date: 08/20/2015, view: 2160, Q&A: 0
    [Abstract] This protocol is a simple colorimetric assay for internal ammonium quantification in aqueous extracts from plant tissues. The method is based on the phenol hypochlorite assay (Berthelot reaction):

    NH4+ + hypochlorite + OH- + phenol → indophenol

    The oxidation of indophenol caused by phenol ...
    Histochemical Detection of Zn in Plant Tissues
    Authors:  Ilya Seregin, Anna Kozhevnikova and Henk Schat, date: 05/20/2015, view: 2818, Q&A: 0
    [Abstract] Accumulation of metals in plant tissues, and occasionally, different cells of the same tissue, may be highly non-uniform (Seregin and Kozhevnikova, 2008). Easy-to-use histochemical methods may greatly help to investigate the distribution and accumulation of metals within and among plant tissues, and also provide information on their subcellular ...
    MIFE Technique-based Screening for Mesophyll K+ Retention for Crop Breeding for Salinity Tolerance
    Authors:  Honghong Wu, Lana Shabala, Meixue Zhou and Sergey Shabala, date: 05/05/2015, view: 2638, Q&A: 0
    [Abstract] Potassium is known as a rate-limiting factor for crop yield and plays an important role in plants response under abiotic stresses. Recently, cytosolic K+ retention ability in leaf mesophyll has emerged as an important component of plant salt tolerance mechanism (Wu et al., 2013; Wu et al., 2014; Wu et al., ...
    A Protocol to Measure the Cytoplasmic Calcium in Arabidopsis Guard Cells
    Authors:  Li Li, Feng Lin, Yana Qu and Qun Zhang, date: 05/05/2015, view: 2904, Q&A: 0
    [Abstract] Cytoplasmic calcium ([Ca2+]cyt) acts as a stimulus-induced second messenger in multiple signal transduction cascades (Allen et al., 1999). In plant cells, a dramatic and readily assayed response to stimulus is the change of stomatal aperture. Changes in [Ca2+]cyt of stomatal guard cells were ...
    Measuring the Arsenic Content and Speciation in Different Rice Tissues
    Authors:  E. Marie Muehe, Andreas Kappler, Christina Chaban and Birgit Daus, date: 04/20/2015, view: 2598, Q&A: 0
    [Abstract] Arsenic (As) plays an important role in rice production as vast soils used for rice cultivation contain As. To understand how rice plants deal with inorganic As (III) and As (V) and organic As in their tissue, it is important to obtain specific information on how much and what species of As are present in which tissue of the rice plant. The ...