Plant Science


    Protocols in Current Issue
    Extraction and Molybdenum Blue-based Quantification of Total Phosphate and Polyphosphate in Parachlorella
    Authors:  Shuhei Ota and Shigeyuki Kawano, date: 09/05/2017, view: 383, Q&A: 0
    [Abstract] Inorganic phosphorus is a non-renewable resource and an essential element for life on Earth. Organisms such as algae, protists, and animals can store phosphate (Pi) through uptake of Pi as polyphosphate (poly-P), which is a linear polymer of orthophosphate residues linked by high-energy phosphoanhydride bonds. Here, we describe procedures for ...
    Determination of Reduced and Total Glutathione Content in Extremophilic Microalga Galdieria phlegrea
    Authors:  Giovanna Salbitani, Claudia Bottone and Simona Carfagna, date: 07/05/2017, view: 717, Q&A: 0
    [Abstract] Glutathione is an important molecule involved in the primary and secondary metabolism of all organisms. The Glutathione redox status is an indicator of the cellular redox state. Therefore, it is important to have precise methods on hand to determine the glutathione redox status in the cell. In this protocol, we describe an improved ...
    Acetyl Bromide Soluble Lignin (ABSL) Assay for Total Lignin Quantification from Plant Biomass
    Authors:  William J. Barnes and Charles T. Anderson, date: 03/05/2017, view: 1626, Q&A: 0
    [Abstract] Lignin is the second most abundant biopolymer on Earth, providing plants with mechanical support in secondary cell walls and defense against abiotic and biotic stresses. However, lignin also acts as a barrier to biomass saccharification for biofuel generation (Carroll and Somerville, 2009; Zhao and Dixon, 2011; Wang et al., 2013). For ...
    Rice Root Organic Acid Efflux Measurement by Using Ion Chromatography
    Authors:  Chun-quan Zhu, Xiao-fang Zhu and Ren-fang Shen, date: 02/20/2017, view: 1421, Q&A: 0
    [Abstract] Organic acids secreted from plant roots play important roles in various biological processes including nutrient acquisition, metal detoxification, and pathogen attraction. The secretion of organic acids may be affected by various conditions such as plant growth stage, nutrient deficiency, and abiotic stress. For example, when white lupin (Lupinus ...
    Chromatographic Separation of the Codonocarpine Type Alkaloids from the Root Bark of Capparis decidua
    Authors:  Yvonne Forster, Abdul Ghaffar and Stefan Bienz, date: 02/20/2017, view: 1238, Q&A: 0
    [Abstract] Various parts of the caper tree Capparis decidua have found application in traditional medicine. The isolation and structural elucidation of the codonocarpine type alkaloids contained in the root bark, however, is not trivial and has probably led to misinterpretation in the past. This protocol describes the extraction and chromatographic ...
    Nitrate Assay for Plant Tissues
    Authors:  Lufei Zhao and Yong Wang, date: 01/20/2017, view: 2332, Q&A: 0
    [Abstract] Nitrogen is an essential macronutrient for plant growth and nitrate content in plants can reflect the nitrogen supply of soil. Here, we provide the salicylic acid method to evaluate the nitrate content in plant tissues. The method is reliable and stable, thus it can be a good choice for measurement of nitrate in plant tissues.
    Cation (Ca2+ and Mn2+) Partitioning Assays with Intact Arabidopsis Chloroplasts
    Authors:  Anna Harms, Iris Steinberger and Anja Schneider, date: 01/05/2017, view: 1675, Q&A: 0
    [Abstract] Determination of the relative distribution of Ca2+ and Mn2+ is an important tool for analyzing mutants showing altered levels of calcium and/or manganese transporters in the chloroplast envelope or thylakoid membrane. The method described in this protocol allows quantitative analyses of the relative distribution of calcium ...
    Quantitative Determination of Ascorbate from the Green Alga Chlamydomonas reinhardtii by HPLC
    Authors:  László Kovács, André Vidal-Meireles, Valéria Nagy and Szilvia Z. Tóth, date: 12/20/2016, view: 1457, Q&A: 0
    [Abstract] Ascorbate (Asc, also called vitamin C) is of vital importance to the cellular functions of both animals and plants. During evolution, Asc has become one of the most abundant metabolites in seed plants; however, Asc contents in cyanobacteria, green algae and bryophytes are very low. Here we describe a sensitive and reliable HPLC method for the ...
    Detection of Reactive Oxygen Species in Oryza sativa L. (Rice)
    Authors:  Navdeep Kaur, Isha Sharma, Kamal Kirat and Pratap Kumar Pati, date: 12/20/2016, view: 2275, Q&A: 0
    [Abstract] Superoxide ions (O2-) and hydrogen peroxide (H2O2) are the reactive oxygen species (ROS) that play a significant role in regulation of many plant processes. The level of O2- ions is determined qualitatively using nitrobluetetrazolium (NBT) assay while the H2O2 is qualitatively ...
    Metabolite Profiling of Mature Arabidopsis thaliana Seeds Using Gas Chromatography-Mass Spectrometry (GC-MS)
    Authors:  Hagai Cohen, Ifat Matityahu and Rachel Amir, date: 11/05/2016, view: 2143, Q&A: 0
    [Abstract] Metabolite profiling using gas chromatography-mass spectrometry (GC-MS) permits the annotation and quantification of a relatively wide variety of metabolites, covering a wide range of biochemical groups of metabolites. Lisec et al. (2006) established a method for GC-MS profiling in plants. Based on this protocol, we provide here a ...