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    Determination of Mn Concentrations in Synechocystis sp. PCC6803 Using ICP-MS
    Authors:  Fabian Brandenburg, Hanan Schoffman, Nir Keren and Marion Eisenhut, date: 12/05/2017, view: 104, Q&A: 0
    [Abstract] Manganese (Mn) is an essential micronutrient for all photoautotrophic organisms. Two distinct pools of Mn have been identified in the cyanobacterium Synechocystis sp. PCC 6803 (Synechocystis), with 80% of the Mn residing in the periplasm and 20% in cytoplasm and thylakoid lumen (Keren et al., 2002). In this protocol, we ...
    Extraction and Molybdenum Blue-based Quantification of Total Phosphate and Polyphosphate in Parachlorella
    Authors:  Shuhei Ota and Shigeyuki Kawano, date: 09/05/2017, view: 663, Q&A: 0
    [Abstract] Inorganic phosphorus is a non-renewable resource and an essential element for life on Earth. Organisms such as algae, protists, and animals can store phosphate (Pi) through uptake of Pi as polyphosphate (poly-P), which is a linear polymer of orthophosphate residues linked by high-energy phosphoanhydride bonds. Here, we describe procedures for ...
    Detection of Reactive Oxygen Species (ROS) in Cyanobacteria Using the Oxidant-sensing Probe 2’,7’-Dichlorodihydrofluorescein Diacetate (DCFH-DA)
    [Abstract] Reactive oxygen species (ROS) are cell signaling molecules synthesized inside the cells as a response to routine metabolic processes. In stress conditions such as ultraviolet radiation (UVR), ROS concentration increases several folds in the cells that become toxic for the cell survival. Here we present the method for in vivo detection of ...
    An ex vivo Perifusion Method for Quantitative Determination of Neuropeptide Release from Mouse Hypothalamic Explants
    Authors:  Ophélia Le Thuc, Jacques Noël and Carole Rovère, date: 08/20/2017, view: 919, Q&A: 0
    [Abstract] The hypothalamus is a primary brain area which, in mammals, regulates several physiological functions that are all related to maintaining general homeostasis, by linking the central nervous system (CNS) and the periphery. The hypothalamus itself can be considered an endocrine brain region of some sort as it hosts in its different nuclei several ...
    Rice Lamina Joint Inclination Assay
    Authors:  Hsing-Yi Li, Hsin-Mei Wang and Seonghoe Jang, date: 07/20/2017, view: 1144, Q&A: 0
    [Abstract] Brassinosteroids (BRs) promote rice lamina inclination. Recently, we showed that OsBUL1 knockout mutant rice (osbul1) is defective in brassinosteroid signaling (Jang et al., 2017). To show that lamina joint inclination of osbul1 is less-sensitive than WT to exogenous brassinolide (BL) treatment in the lamina ...
    Quantitative Determination of Poly-β-hydroxybutyrate in Synechocystis sp. PCC 6803
    Authors:  Yvonne Zilliges and Ramon Damrow, date: 07/20/2017, view: 833, Q&A: 0
    [Abstract] Cyanobacteria synthesize a variety of chemically-different, high-value biopolymers such as glycogen (polyglucose), poly-β-hydroxybutyrate (PHB), cyanophycin (polyamide of arginine and aspartic acid) and volutin (polyphosphate) under excess conditions. Especially under unbalanced C to N ratios, glycogen and in some cyanobacterial genera ...
    Mass Spectrometry-based in vitro Assay to Identify Drugs that Influence Cystine Solubility
    Authors:  Neelanjan Bose, Tiffany Zee, Pankaj Kapahi and Marshall L. Stoller, date: 07/20/2017, view: 959, Q&A: 0
    [Abstract] Cystinuria is a rare genetic disorder characterized by recurrent, painful kidney stones, primarily composed of cystine, the dimer of the amino acid cysteine (Sumorok and Goldfarb, 2013). Using a mouse model of cystinuria, we have recently shown that administration of drugs that increase cystine solubility in the urine can be a novel therapeutic ...
    Endpoint or Kinetic Measurement of Hydrogen Sulfide Production Capacity in Tissue Extracts
    Authors:  Christopher Hine and James R. Mitchell, date: 07/05/2017, view: 1423, Q&A: 0
    [Abstract] Hydrogen sulfide (H2S) gas is produced in cells and tissues via various enzymatic processes. H2S is an important signaling molecule in numerous biological processes, and deficiencies in endogenous H2S production are linked to cardiovascular and other health complications. Quantitation of steady-state H2S ...
    Heavy Metal Stress Assay of Caenorhabditis elegans
    Authors:  Strahil Iv. Pastuhov, Tatsuhiro Shimizu and Naoki Hisamoto, date: 06/05/2017, view: 1420, Q&A: 0
    [Abstract] Organisms have developed many protective systems to reduce the toxicity from heavy metals. The nematode Caenorhabditis elegans has been widely used to determine the protective mechanisms against heavy metals. Responses against heavy metals can be monitored by expression of reporter genes, while sensitivity can be determined by quantifying ...
    Induction and Quantification of Patulin Production in Penicillium Species
    Authors:  Yong Chen, Boqiang Li, Zhanquan Zhang and Shiping Tian, date: 06/05/2017, view: 1135, Q&A: 0
    [Abstract] Patulin, a worldwide regulated mycotoxin, is primarily produced by Penicillium and Aspergillus species during fruit spoilage. Patulin contamination is a great concern with regard to human health because exposure of the mycotoxin can result in severe acute and chronic toxicity, including neurotoxic, mutagenic, and immunotoxic ...