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    Polyamine and Paraquat Transport Assays in Arabidopsis Seedling and Callus
    Authors:  Haoxi Chai, Yun Shen and Huazhong Shi, date: 08/05/2017, view: 331, Q&A: 0
    [Abstract] Polyamines (PAs) are polycationic compounds found in all living organisms and play crucial roles in growth and survival. We here show the ‘Polyamine and paraquat (PQ) transport assay’ protocol, which can be used to examine the uptake activity of PA/PQ transporters. We have used this protocol to demonstrate that PUT3 in Arabidopsis is a ...
    Rice Lamina Joint Inclination Assay
    Authors:  Hsing-Yi Li, Hsin-Mei Wang and Seonghoe Jang, date: 07/20/2017, view: 567, Q&A: 0
    [Abstract] Brassinosteroids (BRs) promote rice lamina inclination. Recently, we showed that OsBUL1 knockout mutant rice (osbul1) is defective in brassinosteroid signaling (Jang et al., 2017). To show that lamina joint inclination of osbul1 is less-sensitive than WT to exogenous brassinolide (BL) treatment in the lamina ...
    Assessing Plant Tolerance to Acute Heat Stress
    Authors:  Minsoo Kim, Fionn McLoughlin, Eman Basha and Elizabeth Vierling, date: 07/20/2017, view: 454, Q&A: 0
    [Abstract] It is well-established that plants are able to acclimate to temperatures above or below the optimal temperature for their growth. Here, we provide protocols for assays that can be used quantitatively or qualitatively to assess the relative ability of plants to acquire tolerance to high temperature stress. The hypocotyl elongation assay described ...
    Non-invasive Protocol for Kinematic Monitoring of Root Growth under Infrared Light
    [Abstract] Phenotyping the dynamics of root responses to environmental cues is necessary to understand plant acclimation to their environment. Continuous monitoring of root growth is challenging because roots normally grow belowground and are very sensitive to their growth environment. This protocol combines infrared imaging with hydroponic cultivation for ...
    Separation of Plant 6-Phosphogluconate Dehydrogenase (6PGDH) Isoforms by Non-denaturing Gel Electrophoresis
    [Abstract] 6-Phosphogluconate dehydrogenase (6PGDH; EC 1.1.1.44) catalyzes the third and irreversible reaction of the pentose phosphate pathway (PPP). It carries out the oxidative decarboxylation of the 6-phosphogluconate to yield ribulose-5-phosphate, carbon dioxide and NADPH. In higher plants, 6PGDH has several subcellular localizations including cytosol, ...
    Determination of the Effects of Local and Systemic Iron Excess on Lateral Root Initiation in Arabidopsis thaliana
    Authors:  Guangjie Li, Lin Zhang and Weiming Shi, date: 07/05/2017, view: 440, Q&A: 0
    [Abstract] Root system architecture depends on nutrient availability. A symptom of iron (Fe) toxicity in plants is stunted root growth, yet little is known about the effects of excess Fe on lateral root (LR) development. To better understand how nutrient signals are integrated into root developmental programs, we investigated the morphological response of ...
    Phototaxis Assay for Chlamydomonas reinhardtii
    Authors:  Noriko Ueki and Ken-ichi Wakabayashi, date: 06/20/2017, view: 758, Q&A: 0
    [Abstract] Phototaxis is a behavior in which organisms move toward or away from the light source (positive or negative phototaxis, respectively). It is crucial for phototrophic microorganisms to inhabit under proper light conditions for phototaxis. The unicellular green alga Chlamydomonas reinhardtii rapidly changes its swimming direction upon light ...
    Estimation of Stomatal Aperture in Arabidopsis thaliana Using Silicone Rubber Imprints
    Authors:  Telma E. Scarpeci, María I. Zanor and Estela M. Valle, date: 06/20/2017, view: 740, Q&A: 0
    [Abstract] Estimation of stomatal aperture using low viscosity silicone-base impression material has the advantage of working with the whole leaf. The developmental stage and the environment strongly affect the stomatal aperture. Therefore, it is mandatory to have accurate estimations of the stomatal aperture of intact leaves under different situations. With ...
    Haustorium Induction Assay of the Parasitic Plant Phtheirospermum japonicum
    Authors:  Juliane K. Ishida, Satoko Yoshida and Ken Shirasu, date: 05/05/2017, view: 860, Q&A: 0
    [Abstract] Phtheirospermum japonicum is a facultative root parasitic plant in the Orobanchaceae family used as a model parasitic plant. Facultative root parasites form an invasive organ called haustorium on the lateral parts of their roots. To functionally characterize parasitic abilities, quantification of haustorium numbers is required. However, ...
    Forest GPP Calculation Using Sap Flow and Water Use Efficiency Measurements
    Authors:  Fyodor Tatarinov, Eyal Rotenberg, Dan Yakir and Tamir Klein, date: 04/20/2017, view: 860, Q&A: 0
    [Abstract] This is a protocol to evaluate gross primary productivity (GPP) of a forest stand based on the measurements of tree’s sap flow (SF), 13C derived water use efficiency (WUE), and meteorological (met) data. GPP was calculated from WUE and stomatal conductance (gs), the later obtained from SF up-scaled from sampled trees to stand ...